关键词:  , 棕背?, 代谢相关脂肪性肝病, 高脂饮食, 动物模型

Abstract: Objective To construct a high-fat diet-induced metabolic associated fatty liver disease (MAFLD) model for Myodes rufocanus, so as to provide a new and reliable animal model for the study of MAFLD. Methods Forty male Myodes rufocanus were randomly divided into two groups and fed normal feed ( control group, NCD) and high-fat feed ( high-fat group, HFD) , respectively. Body weight, blood biochemistry, pathological sections and related gene expression levels were detected in each group at week 8 and week 16 after their death. Result In the high-fat group, Myodes rufocanus were gradually obese, depressed, slow-moving, and had a significantly higher body mass, the liver was enlarged in size, blunted at the edges, and yellowed in color, with a significant increase in the hepatic index, ALT, AST, TG, TC, and LDL-C were significantly higher, and HDL-C was significantly lower, liver sections showed steatosis and deposition. In addition, hepatocytes were filled with lipid droplets and infiltrated with inflammatory cells, with a significant increase in NAS score, the expression levels of lipid synthesis genes ( FASN, SREBF1, ACACA) , inflammation-related genes ( IL-6, TNF-α) , and fibrosis-related genes (α-SMA, TGF-β ) were significantly elevated. Conclusion A successful high-fat diet-induced metabolic associated fatty liver disease (MAFLD) model in Myodes rufocanus has been established, which provides a research basis for relevant studies thereafter.

Key words: Myodes rufocanus, metabolic-associated fatty liver disease, high-fat diets, animal model